DMD Large equally mixed donor pool

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duanmu, Z.
Right arrow Articles by Runge-Morris, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duanmu, Z.
Right arrow Articles by Runge-Morris, M.

Vol. 29, Issue 8, 1130-1135, August 2001

Transcriptional Regulation of Rat Hepatic Aryl Sulfotransferase (SULT1A1) Gene Expression by Glucocorticoids

Zhengbo Duanmu, Thomas A. Kocarek, and Melissa Runge-Morris

Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan

The 5'-flanking region [1892 base pairs (bp)] of the rat aryl sulfotransferase (SULT1A1) gene was cloned and the cis-acting sequences involved in glucocorticoid-inducible SULT1A1 gene transcription were characterized. SULT1A1 promoter and 5'-flanking sequences lacked a TATA box and a consensus glucocorticoid response element. Using a 5'-rapid amplification of cDNA ends approach, four SULT1A1 transcription start sites were identified. Transient transfection studies with SULT1A1-5':luciferase reporter constructs in primary cultured rat hepatocytes revealed that treatment with the potent glucocorticoid dexamethasone (10-9-10-5 M) produced concentration-dependent increases in luciferase activity in constructs containing from 1892 to 119 bp of the SULT1A1 5'-flanking region. Relative to the most upstream SULT1A1 transcription start site, the minimal cis-acting sequences that were required for dexamethasone-inducible SULT1A1 expression were located between -84 and -69 bp. Treatment of transfectants with a panel of steroids, including dexamethasone, triamcinolone acetonide, hydrocortisone, dihydrotestosterone, 17beta -estradiol, and pregnenolone-16alpha -carbonitrile, revealed that steroid-inducible SULT1A1 gene expression was specific for glucocorticoid-class steroids. Concentration-response studies, coupled with a robust inhibition of glucocorticoid-inducible SULT1A1-5':luciferase reporter activity by antiglucocorticoid/antiprogestin RU-486, recapitulated earlier findings on endogenous SULT1A1 gene expression and implicated a major role for the glucocorticoid receptor transcription factor in the regulation of glucocorticoid-inducible SULT1A1 gene expression.

Copyright © 2001 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Alnouti and C. D. Klaassen
Regulation of Sulfotransferase Enzymes by Prototypical Microsomal Enzyme Inducers in Mice
J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 612 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
R. Pastorelli, D. Carpi, R. Campagna, L. Airoldi, R. Pohjanvirta, M. Viluksela, H. Hakansson, P. C. Boutros, I. D. Moffat, A. B. Okey, et al.
Differential Expression Profiling of the Hepatic Proteome in a Rat Model of Dioxin Resistance: CORRELATION WITH GENOMIC AND TRANSCRIPTOMIC ANALYSES
Mol. Cell. Proteomics, May 1, 2006; 5(5): 882 - 894.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C.-T. Yeh and G.-C. Yen
Involvement of p38 MAPK and Nrf2 in phenolic acid-induced P-form phenol sulfotransferase expression in human hepatoma HepG2 cells
Carcinogenesis, May 1, 2006; 27(5): 1008 - 1017.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
U. A. Nuber, S. Kriaucionis, T. C. Roloff, J. Guy, J. Selfridge, C. Steinhoff, R. Schulz, B. Lipkowitz, H. H. Ropers, M. C. Holmes, et al.
Up-regulation of glucocorticoid-regulated genes in a mouse model of Rett syndrome
Hum. Mol. Genet., August 1, 2005; 14(15): 2247 - 2256.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
N. Hempel, H. Wang, E. L. LeCluyse, M. E. McManus, and M. Negishi
The Human Sulfotransferase SULT1A1 Gene Is Regulated in a Synergistic Manner by Sp1 and GA Binding Protein
Mol. Pharmacol., December 1, 2004; 66(6): 1690 - 1701.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
D. P. Hartley, X. Dai, Y. D. He, E. J. Carlini, B. Wang, S.-e. W. Huskey, R. G. Ulrich, T. H. Rushmore, R. Evers, and D. C. Evans
Activators of the Rat Pregnane X Receptor Differentially Modulate Hepatic and Intestinal Gene Expression
Mol. Pharmacol., May 1, 2004; 65(5): 1159 - 1171.
[Abstract] [Full Text]

Home page
 Drug Metab. Dispos.Home page
H.-L. Fang, S. Shenoy, Z. Duanmu, T. A. Kocarek, and M. Runge-Morris
TRANSACTIVATION OF GLUCOCORTICOID-INDUCIBLE RAT ARYL SULFOTRANSFERASE (SULT1A1) GENE TRANSCRIPTION
Drug Metab. Dispos., November 1, 2003; 31(11): 1378 - 1381.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
Z. Duanmu, D. Locke, J. Smigelski, W. Wu, M. S. Dahn, C. N. Falany, T. A. Kocarek, and M. Runge-Morris
Effects of Dexamethasone on Aryl (SULT1A1)- and Hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes
Drug Metab. Dispos., September 1, 2002; 30(9): 997 - 1004.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2001 by the American Society for Pharmacology and Experimental Therapeutics.