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0090-9556/03/3106-691-693$20.00
DMD 31:691-693, 2003

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SHORT COMMUNICATION

BASOLATERAL ACTIVE UPTAKE OF NITROFURANTOIN IN THE CIT3 CELL CULTURE MODEL OF LACTATION


Phillip M. Gerk
Jeffrey A. Moscow
Patrick J. McNamara

University of Kentucky College of Pharmacy, Division of Pharmaceutical Sciences (P.M.G., P.J.M.); and University of Kentucky College of Medicine, Department of Pediatrics (J.A.M.), Lexington, Kentucky

Nitrofurantoin and other agents are actively transported into human and rodent milk. The purpose of this study was to determine whether nitrofurantoin active transport across mammary epithelia occurs basolaterally or apically, using the CIT3 cell culture model of lactation. The CIT3 model actively transports nitrofurantoin in the basolateral to apical direction. Basolateral to apical permeability [92.9 ± 6.6 (µl/h)/cm2] was differentially decreased by unlabeled nitrofurantoin (250 µM) on the basolateral, apical, or both sides [49.5 ± 1.8, 57.9 ± 1.4, or 48.5 ± 1.6 (µl/h)/cm2, respectively]. Apical to basolateral permeability [27.6 ± 1.8 (µl/h)/cm2] was increased in the presence of unlabeled nitrofurantoin (250 µM) on the basolateral, apical, or both sides [36.4 ± 1.5, 39.9 ± 0.7, 42.4 ± 1.1 (µl/h)/cm2, respectively]. These data indicate a basolateral active uptake mechanism for nitrofurantoin, which remains to be identified. This mechanism may influence the exposure of suckling infants to xenobiotics, as well as having potentially toxic effects on the lactating mammary epithelium and possibly altering the nutritional quality of the milk.

Address correspondence to: Dr. Patrick J. McNamara, Room 401A Pharmacy Building, University of Kentucky College of Pharmacy, Lexington, KY 40536-0082. E-mail: pmcnamar{at}email.uky.edu




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