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0090-9556/03/3106-701-704$20.00
DMD 31:701-704, 2003

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SHORT COMMUNICATION

QUINOL-BASED METABOLIC CYCLE FOR ESTROGENS IN RAT LIVER MICROSOMES


Laszlo Prokai
Katalin Prokai-Tatrai
Pal Perjesi
Alevtina D. Zharikova
James W. Simpkins

Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, Florida (L.P., P.P., A.D.Z.); Center for Neurobiology of Aging, College of Medicine, University of Florida, Gainesville, Florida (K.P.-T.); Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas (J.W.S.)

According to a recently reported metabolic pathway, phenolic A-ring estrogens are metabolized in rat liver microsomes partially to the corresponding quinols by cytochrome P450 isoenzymes. We found that these quinols could, in turn, undergo reduction to regenerate the parent estrogens consumed during the metabolic process. Among the tested endogenous reducing agents, NADH and especially NADPH produced a significant extent of reductive conversion. Enzymes available in rat liver microsomes further catalyzed this reaction with 6.5 ± 1.5 nmol · min-1 · (mg of protein)-1 measured as the initial rate of estrone formation at 37°C, whereas the initial rate of second-order reaction for the reduction of E1-quinol by a 10-fold excess of NADPH in a microsome-free buffer solution and under identical incubation conditions was 0.62 ± 0.03 nmol · min-1. The quinol route is, therefore, unique among estrogen-metabolizing pathways for its bioreversibility due to the facile regeneration of the phenolic A-ring estrogens consumed in the preceding oxidative process.

Address correspondence to: Dr. Laszlo Prokai, Department of Medicinal Chemistry, College of Pharmacy, 1600 SW Archer Road, University of Florida, Gainesville, Florida 32610-0485. E-mail: lprokai{at}grove.ufl.edu




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