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Drug Metabolism and Disposition Fast Forward
First published on June 2, 2006; DOI: 10.1124/dmd.106.010777

0090-9556/06/3409-1433-1435$20.00
DMD 34:1433-1435, 2006

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SHORT COMMUNICATION

A Theoretical Validation of the Substrate Depletion Approach to Determining Kinetic Parameters

Abhinav Nath, and William M. Atkins

Department of Medicinal Chemistry, University of Washington, Seattle, Washington

The substrate depletion approach is an increasingly popular alternative to the traditional method (observing product formation) of determining the kinetic parameters (KM and Vmax) of an enzyme. Obach and Reed-Hagen (Drug Metab Dispos 30:831–837, 2002) used an empirical relationship between substrate depletion rate constants and initial substrate concentration to determine kinetic parameters for a number of cytochrome P450-catalyzed reactions. We present a proof that this relationship can be derived from the Michaelis-Menten equation and, therefore, that kinetic parameters obtained by the substrate depletion approach are equivalent and comparable to those obtained by the traditional product formation approach. Analysis of a simulated data set produced similar kinetic parameters regardless of which approach was used, confirming the theoretical result.

Address correspondence to: William M. Atkins, Department of Medicinal Chemistry, Box 357610, University of Washington, Seattle, WA 91895-7610. E-mail: winky{at}u.washington.edu




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