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Drug Metabolism and Disposition Fast Forward
First published on November 5, 2007; DOI: 10.1124/dmd.107.017632

0090-9556/08/3602-316-321$20.00
DMD 36:316-321, 2008

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Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription

Ruchita Kapoor, and Jonathan J. Sheng

Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, North Dakota

Human cytosolic sulfotransferase SULT1E1 catalyzes the sulfation of estrogens and estrogenic drugs in human reproductive tissues. Logically, this estrogen-preferring sulfotransferase isoform could play a regulatory role in estrogen signaling activities in human reproductive cells, including the prostate cells. This hypothesis was tested using DNA microarray and real-time reverse transcription-polymerase chain reaction methods in the present work. Potential changes in the transcriptional expression of selected signal transduction-related genes in human prostate cancer CA-HPV-10 cell line after SULT1E1 transfection were examined by DNA microarray methods. Notable changes were observed in the mRNA expression levels of TFRC, a cell membrane transferrin receptor gene, and TMEPAI, a gene encoding a steroid-dependent mRNA product. Expression of TFRC was down-regulated, whereas expression of TMEPAI was up-regulated by SULT1E1 transfection in CA-HPV-10 cells. Data from the current studies also showed that the estrogen-induced estrogen response element activation in CA-HPV-10 cells was repressed after the cells were transfected with SULT1E1. These results indicate that SULT1E1 may function as a transcriptional mediator in human prostate cancer CA-HPV-10 cells.

Address correspondence to: Jonathan J. Sheng, Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, ND 58105. E-mail: jonathan.sheng{at}ndsu.edu






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