The drawing is a representation of the final complex formed in the assay. The long solid black line represents the single-stranded mRNA target, blue lines represent capture probes, red lines represent label probes, and green lines represent blocker probes. Oligonucleotides complementary to the tail of the capture probes are shown as short black bars attached to the solid support phase. The bDNA molecule (light blue), with its 15 branch points, is shown hybridized to the tail of the label probe. Alkaline phosphatase (AP; red diamonds) conjugated oligonucleotides (orange) are shown hybridized to the branches of the bDNA molecule (3 AP-oligonucleotide conjugate molecules/branch). The signal generated by AP-mediated dioxetane chemiluminescence is shown in yellow.

Each single-stranded CYP mRNA transcript is represented by a black line, blocker probes are represented by green lines, capture probes are represented by blue lines, label probes are represented by red lines, and gaps in the probe set are indicated by arrows and the nucleotide position at which the gap occurs.