QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content

ERRATUM

Correction to "Stereoselective Metabolism of Endosulfan by Human Liver Microsomes and Human Cytochrome P540 Isoforms"

On page 1090, the sentence "The P450 isoform-selective inhibition study in human liver microsomes and the incubation study of cDNA-expressed enzymes also demonstrated that the stereoselective sulfonation of -endosulfan is mediated by CYP2B6, CYP3A4, and CYP3A5, and that that of ß-endosulfan is transformed by CYP3A4 and CYP3A5" should be corrected to: "The P450 isoform-selective inhibition study in human liver microsomes and the incubation study of cDNA-expressed enzymes also demonstrated that the stereoselective endosulfan sulfate formation from -endosulfan is mediated by CYP2B6, CYP3A4, and CYP3A5, and that from ß-endosulfan is transformed by CYP3A4 and CYP3A5".

On page 1090, the sentence "CYP2B6 enantioselectively metabolizes -endosulfan, but not ß-endosulfan" should be corrected to: "CYP2B6 stereoselectively metabolizes -endosulfan, but not ß-endosulfan".

On page 1091, the sentence "Metabolism of Endosulfan Enantiomers in Human Liver Microsomes or cDNA-Expressed P450 Isoforms" should be corrected to: "Metabolism of Endosulfan Isomers in Human Liver Microsomes or cDNA-Expressed P450 Isoforms".

On page 1091, the sentence "The reactions were initiated by the addition of various concentrations of endosulfan enantiomer (0.5–100 µM)" should be corrected to: "The reactions were initiated by the addition of various concentrations of endosulfan isomer (0.5–100 µM)".

On page 1091, the sentence "Endosulfan enantiomers (10 µM) were incubated with microsomes from 14 different livers" should be corrected to: "Endosulfan isomers (10 µM) were incubated with microsomes from 14 different livers".

On page 1091, the sentence "Contributions of each cytochrome to endosulfan sulfonation were normalized for mean values of the relative abundance of individual cytochromes in the liver" should be corrected to: "Contributions of each cytochrome to endosulfan sulfate formation were normalized for mean values of the relative abundance of individual cytochromes in the liver".

On page 1092 (Fig. 2), the sentence "Stereoselective inhibition of known P450 isoform-selective inhibitors on sulfonation from -endosulfan (A) and ß-endosulfan (B) in human liver microsomal incubations" should be corrected to: "Stereoselective inhibition of known P450 isoform-selective inhibitors on endosulfan sulfate formation from -endosulfan (A) and ß-endosulfan (B) in human liver microsomal incubations".

On page 1092 (Fig. 2), the sentence "Pooled human liver microsomes (0.2 mg/ml; BD Gentest) were incubated with 10 µM endosulfan enantiomers in the absence or presence of various chemical inhibitors at 37°C for 30 min" should be corrected to: "Pooled human liver microsomes (0.2 mg/ml; BD Gentest) were incubated with 10 µM endosulfan isomers in the absence or presence of various chemical inhibitors at 37°C for 30 min".

On page 1092 (Table 1), the sentence "Correlation of formation rates of endosulfan sulfate from endosulfan enantiomers (10 µM) with the P450 marker activities in human liver microsomes (n = 14)" should be corrected to: "Correlation of formation rates of endosulfan sulfate from endosulfan isomers (10 µM) with the P450 marker activities in human liver microsomes (n = 14)".

On page 1092, the sentence "Among the nine inhibitors tested, ketoconazole, a well known CYP3A-selective inhibitor, inhibited endosulfan sulfate formation from both enantiomers" should be corrected to: "Among the nine inhibitors tested, ketoconazole, a well known CYP3A-selective inhibitor, inhibited endosulfan sulfate formation from both isomers".

On page 1092, the sentence "Endosulfan sulfate formation from -endosulfan was also inhibited by thio-TEPA as well as ketoconazole, suggesting that the stereoselective sulfonation of -endosulfan is mediated by more than one enzyme" should be corrected to: "Endosulfan sulfate formation from -endosulfan was also inhibited by thio-TEPA as well as ketoconazole, suggesting that the stereoselective endosulfan sulfate formation from -endosulfan is mediated by more than one enzyme".

On page 1092, the sentence "Endosulfan sulfate formation from endosulfan enantiomers was also studied using the human cDNA-expressed P450 isoforms" should be corrected to: "Endosulfan sulfate formation from endosulfan isomers was also studied using the human cDNA-expressed P450 isoforms".

On page 1093 (Fig. 5), the sentence "Representative plot of the formation of endosulfan sulfate from endosulfan enantiomers by cDNA-expressed P450 isoforms" should be corrected to: "Representative plot of the formation of endosulfan sulfate from endosulfan isomers by cDNA-expressed P450 isoforms".

On page 1093 (Fig. 5), the sentence "Human cDNA-expressed CYP1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4, and 3A5 were incubated with 10 µM endosulfan enantiomers at 37°C for 30 min" should be corrected to: "Human cDNA-expressed CYP1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4, and 3A5 were incubated with 10 µM endosulfan isomers at 37°C for 30 min".

On page 1093 (Fig. 6), the sentence "Kinetics for the formation rate of endosulfan sulfate from endosulfan enantiomers in human liver microsomes" should be corrected to: "Kinetics for the formation rate of endosulfan sulfate from endosulfan isomers in human liver microsomes".

On page 1093, the sentence "The cDNA-expressed CYP2B6, however, catalyzed endosulfan sulfate formation with a higher formation rate from the -enantiomer than from the ß-form" should be corrected to: "The cDNA-expressed CYP2B6, however, catalyzed endosulfan sulfate formation with a higher formation rate from -endosulfan than from ß-endosulfan".

On page 1093, the sentence "CYP3A4 and CYP3A5 metabolized both endosulfan enantiomers to endosulfan sulfate" should be corrected to: "CYP3A4 and CYP3A5 oxidized both endosulfan isomers to endosulfan sulfate".

On page 1093, the sentence "The CYP3A4-catalyzed endosulfan sulfate formation of ß-endosulfan showed a higher V_max and K_m than those of the -enantiomer" should be corrected to: "The CYP3A4-catalyzed endosulfan sulfate formation of ß-endosulfan showed a higher V_max and K_m than those of -endosulfan".

On page 1094, the sentence "the rates of formation of endosulfan sulfate from endosulfan enantiomers also correlated very well with the CYP3A activity, as shown in Table 1" should be corrected to: "the rates of formation of endosulfan sulfate from endosulfan isomers also correlated very well with the CYP3A activity, as shown in Table 1".

On page 1094, the sentence "In the inhibition study with sulfaphenazole, a selective CYP2C9 inhibitor, -endosulfan sulfonation was slightly inhibited (30%) by sulfaphenazole" should be corrected to: "In the inhibition study with sulfaphenazole, a selective CYP2C9 inhibitor, endosulfan sulfate formation from -endosulfan was slightly inhibited (30%) by sulfaphenazole".

On page 1094, the sentence "Therefore, the slight inhibition of -endosulfan sulfonation might be a minor inhibitory effect of CYP2B6 by sulfaphenazole" should be corrected to: "Therefore, the slight inhibition of endosulfan sulfate formation from -endosulfan[/underln] might be a minor inhibitory effect of CYP2B6 by sulfaphenazole".

On page 1094, the sentence "Taken together, these results suggest that CYP3A4 is the major enzyme involved in the sulfonation of both endosulfan enantiomers at concentrations in the usual experimental range" should be corrected to: "Taken together, these results suggest that CYP3A4 is the major enzyme involved in endosulfan sulfate formation from both endosulfan isomers at concentrations in the usual experimental range".

On page 1094 (Fig. 7), the sentence "The formation of endosulfan sulfate from endosulfan enantiomers in recombinant human CYP2B6 (A), CYP3A4 (B), and CYP3A5 (C)" should be corrected to: "The formation of endosulfan sulfate from endosulfan isomers in recombinant human CYP2B6 (A), CYP3A4 (B), and CYP3A5 (C)".

On page 1094 (Fig. 8), the sentence "Abundance-adjusted simulations of the relative contributions of three or two P450 isozymes to endosulfan sulfonation in relation to - and ß-endosulfan concentrations" should be corrected to: "Abundance-adjusted simulations of the relative contributions of three or two P450 isozymes to endosulfan sulfate formation in relation to - and ß-endosulfan concentrations".

On page 1095, the sentence "in which CYP3A4 was shown to have a high degree of sulfonation activity" should be corrected to: "in which CYP3A4 was shown to have a high degree of endosulfan sulfate formation activity".

On page 1095, the sentence "Hence, subjects with a high liver CYP2B6 level would be expected to exhibit higher -endosulfan sulfonation by CYP2B6 than by CYP3A4" should be corrected to: "Hence, subjects with a high liver CYP2B6 level would be expected to exhibit higher -endosulfan oxidation by CYP2B6 than by CYP3A4".

On page 1095, the sentence "CYP2B6 metabolized -endosulfan, but not ß-endosulfan, in an enantio-selective manner, and that CYP3A4 and CYP3A5 were responsible for the enantioselective formation of endosulfan sulfate from both isomers" should be corrected to: "CYP2B6 selectively oxidized -endosulfan, but not ß-endosulfan, and that CYP3A4 and CYP3A5 were responsible for the formation of endosulfan sulfate from both isomers".

The online version of this article has been corrected in departure from the print version.

The authors apologize for any confusion and inconveniences caused by these errors, and greatly appreciate the kind and valuable comments of Dr. Joseph Gal for correction of these errors.

This Article Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content