DMD Simcyp

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

0090-9556//0--$20.00
DMD :-,

This Article
Right arrow Abstract
Right arrow Full Text
Right arrow Submit a response
Services
Right arrow Alert me to new issues of the journal
CHARACTERIZATION OF TRANSPORT PROTEIN EXPRESSION IN MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN (MRP) 2-DEFICIENT RATS
Drug Metab Dispos Johnson et al. 34: 556

Authors' Note

While this manuscript was undergoing review, Chen et al. (2005) published results demonstrating a 200% increase in Mrp4 protein expression in the kidney of TR- rats when compared to wild-type controls. Based on experiments conducted in our laboratory, the polyclonal Mrp4 antibody detailed by Leggas et al. (2004) provided similar results to a commercially available monoclonal antibody to MRP4 (M4I-10, Alexis Biochemicals, San Diego, CA). Our laboratory then repeated Mrp4 Western blot experiments in kidney tissue of TR- and wild-type rats (n=3 per group) using the polyclonal Mrp4 antibody detailed by Leggas et al. (2004). Mrp4 protein expression in TR- rats was slightly higher than that observed in wild-type controls; however, expression levels were not statistically different when normalized for b-actin. The apparent discrepancy in these results may be due to differences in experimental methodology and/or housing/dietary factors.
Chen C, Slitt AL, Dieter MZ, Tanaka Y, Scheffer GL, Klaassen CD (2005) Up-regulation of Mrp4 expression in kidney of Mrp2-deficient TR- rats. Biochem Pharmacol 70:1088-1095.





This Article
Right arrow Abstract
Right arrow Full Text
Right arrow Submit a response
Services
Right arrow Alert me to new issues of the journal

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition