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Drug Metabolism and Disposition Fast Forward
First published on May 30, 2007; DOI: 10.1124/dmd.106.014605

0090-9556/07/3509-1533-1542$20.00
DMD 35:1533-1542, 2007

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ABCG2 (Breast Cancer Resistance Protein/Mitoxantrone Resistance-Associated Protein) ATPase Assay: A Useful Tool to Detect Drug-Transporter Interactions

Hristos Glavinas, Emese Kis, Ákos Pál, Rita Kovács, Márton Jani, Erika Vági, Éva Molnár, Száva Bánsághi, Zoltán Kele, Tamás Janáky, György Báthori, Oliver von Richter, Gerrit-Jan Koomen, and Péter Krajcsi

SOLVO Biotechnology, Central Hungarian Innovations Center, Budaörs, Hungary (H.G., E.K., Á.P., R.K., P.K., M.J., E.V., É.M., S.B., G.B.); Department of Medicinal Chemistry, University of Szeged Medical School, Szeged, Hungary (Z.K., T.J.); Division of Drug Metabolism and Pharmacokinetics, ALTANA Pharma AG, Konstanz, Germany (O.v.R.); Bioorganic Chemistry, IMC, University of Amsterdam, Amsterdam, The Netherlands (G.-J.K.); and Rational Drug Design Laboratories, Budapest, Hungary (P.K.)

The ATPase assay using membrane preparations from recombinant baculovirus-infected Spodoptera frugiperda ovarian (Sf9) cells is widely used to detect the interaction of compounds with different ATP-binding cassette transporters. However, Sf9 membrane preparations containing the wild-type ABCG2 transporter show an elevated baseline vanadate-sensitive ATPase activity, which cannot be further stimulated by substrates of ABCG2. Therefore, this assay system cannot be used for the detection of ABCG2 substrates. To overcome this difficulty we 1) purified membranes from a selected human cell line expressing wild-type ABCG2, and 2) inhibited the baseline ATPase activity with different inhibitors. In our modified assay, ABCG2 substrates were able to stimulate the baseline ATPase activity of ABCG2 expressed in membranes of human cells. Furthermore, using the specific ABCG2 inhibitors Ko143 or Ko134 allowed us to suppress the baseline vanadate-sensitive ATPase activity. Substrates of ABCG2 could stimulate this suppressed baseline ATPase, resulting in a better signal-to-background ratio and a robust assay to detect substrates of the ABCG2 transporter. The ATPase assay and the direct vesicular transport measurements for estrone-3-sulfate were in good accordance.

Address correspondence to: Peter Krajcsi, SOLVO Biotechnology Inc. Central Hungarian Innovations Center, Gyár u. 2, H-2040 Budaörs, Hungary. E-mail: krajcsi{at}solvo.com






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