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Drug Metabolism and Disposition Fast Forward
First published on July 21, 2004; DOI: 10.1124/dmd.104.000620

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Received for publication May 14, 2004.
Revised July 15, 2004.
Accepted for publication July 16, 2004.

Identification of di (2-ethylhexyl) phthalate-induced carboxylesterase 1 in C57BL/6 mouse liver microsomes: Purification, cDNA cloning, and baculovirus-mediated expression

Tomomi Furihata 1, Masakiyo Hosokawa 1*, Nao Koyano 1, Takahiro Nakamura 1, Tetsuo Satoh 1, Kan Chiba 1

1 Chiba University

* Address correspondence to: E-mail: masakiyo{at}p.chiba-u.ac.jp

Abstract

Several mouse carboxylesterase (CES) isozymes have been identified, but information about their roles in drug metabolism is limited. In this study, we purified and characterized a mouse CES1 isozyme that was induced by di (2-ethylhexyl) phthalate. Purified mouse CES1 shared some biological characteristics with other CES isozymes, such as molecular weight of a subunit and isoelectronic point. In addition, purified mouse CES1 behaved as a trimer, a specific characteristic of CES1A subfamily isozymes. The purified enzyme possessed temocapril hydrolase activity and it was found to contribute significantly to temocapril hydrolase activity in mouse liver microsomes. To identify the nucleotide sequences coding mouse CES1, antibody screening of a cDNA library was performed. The deduced amino acid sequence of the obtained cDNA, mCES1, exhibited striking similarity to those of CES1A isozymes. When expressed in Sf9 cells, recombinant mCES1 showed hydrolytic activity towards temocapril, as did purified mouse CES1. Based on these results together with the findings that recombinant mouse CES1 had the same molecular weight of a subunit, the same isoelectronic point and the same native protein mass as those of purified mouse CES1, it was concluded that mCES1 encoded mouse CES1. Furthermore, tissue expression profiles of mCES1 were found to be very similar to those of the human CES1 isozyme. This finding, together with our other results, suggests that mCES1 shares many biological properties with the human CES1 isozyme. The present study has provided useful information for study of metabolism and dispositions of ester-prodrugs as well as ester-drugs.


Key words: carboxylesterases, induction, liver microsomes, prodrugs


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