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Drug Metabolism and Disposition Fast Forward
First published on October 1, 2004; DOI: 10.1124/dmd.104.001917

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Received for publication August 20, 2004.
Revised September 24, 2004.
Accepted for publication September 28, 2004.

Lack of evidence for induction of CYP2B1, CYP3A23, and CYP1A2 gene expression by Panax ginseng and Panax quinquefolius extracts in adult rats and primary cultures of rat hepatocytes

Chia-Ting Yu 1, Jie Chen 1, Xiao Wei Teng 1, Vincent Tong 1, Thomas K. H. Chang 1*

1 University of British Columbia

* Address correspondence to: E-mail: tchang{at}interchange.ubc.ca

Abstract

Treatment of rats with a single oral dose (10-30 mg/kg) of a crude Panax ginseng extract of unknown ginsenoside content has been reported to modestly increase hepatic microsomal cytochrome P450-mediated aminopyrine N-demethylation activity. In the present study, we compared the effect of Panax ginseng and Panax quinquefolius extracts on rat hepatic CYP2B1, CYP3A23, and CYP1A2 gene expression. Adult male Sprague-Dawley rats (250-275 g) were administered by oral gavage or i.p. with Panax ginseng extract (4% w/w total ginsenosides; 30 or 100 mg/kg/day for 1 or 4 days), Panax quinquefolius extract (10% w/w total ginsenosides; 100 or 400 mg/kg/day for 21 consecutive days), or an equivalent volume (2 ml/kg) of the vehicle (0.9% NaCl or 0.3% carboxymethylcellulose) and terminated one day after the last dose. Panax ginseng and Panax quinquefolius extracts did not affect body weight gain, absolute or relative liver weight, hepatic CYP2B1, CYP3A23, or CYP1A2 mRNA expression, or microsomal CYP2B-mediated 7-benzyloxyresorufin O-dealkylation (BROD) or CYP1A-mediated 7-ethoxyresorufin O-dealkylation (EROD) activity. In contrast, results from positive control experiments indicated that phenobarbital increased CYP2B1 mRNA and BROD activity, dexamethasone increased CYP3A23 mRNA, and beta-naphthoflavone increased CYP1A2 mRNA and EROD activity levels. Treatment of primary cultures of rat hepatocytes with either of the ginseng extracts (0.1-1000 µg/ml for 2 days) also did not affect CYP2B1 or CYP3A23 mRNA expression. Overall, our data indicate that Panax ginseng and Panax quinquefolius extracts do not increase rat hepatic CYP2B1, CYP3A23, or CYP1A2 gene expression.


Key words: CYP induction, CYP1A, CYP2B, CYP3A, cytochrome P450


This article has been cited by other articles:


Home page
 Drug Metab. Dispos.Home page
T. K. H. Chang, J. Chen, and X. W. Teng
DISTINCT ROLE OF BILOBALIDE AND GINKGOLIDE A IN THE MODULATION OF RAT CYP2B1 AND CYP3A23 GENE EXPRESSION BY GINKGO BILOBA EXTRACT IN CULTURED HEPATOCYTES
Drug Metab. Dispos., February 1, 2006; 34(2): 234 - 242.
[Abstract] [Full Text] [PDF]


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