DMD Noab BioDiscoveries - Shaping Drug Discovery

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

Drug Metabolism and Disposition Fast Forward
First published on April 15, 2005; DOI: 10.1124/dmd.104.002691

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dmd.104.002691v1
33/7/1036    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Author home page(s):
Christine Ann Edwards
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Graf, B. A
Right arrow Articles by Edwards, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Graf, B. A
Right arrow Articles by Edwards, C. A.


Received for publication November 2, 2004.
Revised April 11, 2005.
Accepted for publication April 12, 2005.

DISPOSITION AND METABOLISM OF [2-14C]QUERCETIN-4'-GLUCOSIDE IN RATS

Brigitte A Graf 1, William Mullen 1, Caldwell T Stewart 2, Richard C Hartley 1, Gary G Duthie 3, Michael EJ Lean 1, Alan Crozier 1, Christine Ann Edwards 1*

1 Glasgow University 2 University of Glasgow 3 Rowett Research Institute

* Address correspondence to: E-mail: cae1n{at}clinmed.gla.ac.uk

Abstract

Quercetin-4'-glucoside is a major flavonol in onions and this study investigated the absorption and fate of radiolabelled quercetin-4'-glucoside in rats. Rats ingested [2-14C]quercetin-4'-glucoside and the distribution of radioactivity throughout the body was determined after 0.5, 1, 2 and 5 h. The gastrointestinal tract, liver, kidney and plasma were extracted and radiolabelled components identified and quantified using high performance liquid chromatography with on-line radioactivity detection and tandem mass spectrometry. Two hours after dosing all the [2-14C]quercetin-4'-glucoside had been metabolised. Over 85% of the ingested radioactivity was present in the gastrointestinal-tract at all time points with ca. 6% being absorbed and present in blood and internal organs, primarily the liver and kidneys. More than 95% of the absorbed radioactivity was in the form of >20 different methylated glucuronated and/or sulphated quercetin conjugates. Five hours after ingestion, the main radiolabelled metabolites were quercetin di-glucuronides in the gut, liver, kidney, and glucuronyl sulphates of methylated quercetin in plasma. The main site of quercetin metabolism appeared to be the gastrointestinal tract. Quercetin metabolites may have a major influence on the gut mucosal epithelium and on colonic disease.


Key words: absorption, antioxidants, intestinal bioavailability, physiologically-based modeling


This article has been cited by other articles:


Home page
 J. Nutr.Home page
W. Wiczkowski, J. Romaszko, A. Bucinski, D. Szawara-Nowak, J. Honke, H. Zielinski, and M. K. Piskula
Quercetin from Shallots (Allium cepa L. var. aggregatum) Is More Bioavailable Than Its Glucosides
J. Nutr., May 1, 2008; 138(5): 885 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
B. A. Graf, C. Ameho, G. G. Dolnikowski, P. E. Milbury, C.-Y. Chen, and J. B. Blumberg
Rat Gastrointestinal Tissues Metabolize Quercetin
J. Nutr., January 1, 2006; 136(1): 39 - 44.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2005 by the American Society for Pharmacology and Experimental Therapeutics.