DMD Celsis microsomes equal better data

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

Drug Metabolism and Disposition Fast Forward
First published on December 28, 2005; DOI: 10.1124/dmd.105.006932

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dmd.105.006932v1
34/3/477    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alnouti, Y.
Right arrow Articles by Klaassen, C. D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alnouti, Y.
Right arrow Articles by Klaassen, C. D


Received for publication August 22, 2005.
Revised December 12, 2005.
Accepted for publication December 14, 2005.

Tissue Distribution and Ontogeny of Organic Cation Transporters in Mice

Yazen Alnouti 1, Jay S Petrick 1, Curtis D Klaassen 1*

1 University of Kansas Medical Center

* Address correspondence to: E-mail: cklassee{at}kumc.edu

Abstract

Organic cation transporters (Octs) play an important role in transporting cationic xeno- and endobiotics across biological membranes. Little is known about Octs in mice, therefore the tissue distribution and developmental changes in the mRNA expression of Octs in mice were quantified. Oct1, Oct2, Oct3, Octn1, Octn2, and Octn3 mRNA expression was quantified in 14 tissues from male and female mice using the branched DNA signal amplification assay (bDNA). Oct1 mRNA expression was highest in kidney, followed by liver. Oct2 mRNA was almost exclusively expressed in kidney, with male mice having twice that in female mice. The higher expression of Oct2 in male mice is due to testosterone. Oct3 mRNA was most highly expressed in placenta, ovary, and uterus, but was expressed at low levels in most tissues. Octn1 and Octn2 mRNA expression was similar, with highest levels in kidney followed by small intestine. Octn3 mRNA was almost exclusively expressed in testes. The developmental expression of Oct1, Oct2, Octn1, and Octn2 mRNA in kidneys as well as Oct1 in livers was determined in young mice. Ontogenic expression data indicate that each of the Oct transporters approached adult expression levels by about three weeks of age. The gender difference in Oct mRNA expression did not become apparent until day 30 after birth. The differences in tissue distribution of the Octs may play an important role in drug disposition to various tissues. Furthermore, low expression of the Octs in young animals may affect the pharmacokinetic behavior of drugs compared to that in adults.


Key words: active transport, distribution, organic cation transport, transporters


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
N. L. Baganz, R. E. Horton, A. S. Calderon, W. A. Owens, J. L. Munn, L. T. Watts, N. Koldzic-Zivanovic, N. A. Jeske, W. Koek, G. M. Toney, et al.
Organic cation transporter 3: Keeping the brake on extracellular serotonin in serotonin-transporter-deficient mice
PNAS, December 2, 2008; 105(48): 18976 - 18981.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. K. Filipski, W. J. Loos, J. Verweij, and A. Sparreboom
Interaction of Cisplatin with the Human Organic Cation Transporter 2
Clin. Cancer Res., June 15, 2008; 14(12): 3875 - 3880.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2005 by the American Society for Pharmacology and Experimental Therapeutics.