Abstract
3H-Digitoxigenin was incubated over a time course of 15, 30, 45, and 60 min with liver homogenates prepared from phenobarbital-pretreated and control male rabbits. Pretreatment with phenobarbital preferentially enhanced the formation of 6β-hydroxy-3-epidigitoxigenin at all time points and significantly decreased the accumulation of 3-epidigitoxigenin at 45 and 60 min. The formation of 5β-hydroxydigitoxigenin was significantly greater at 15, 30, and 45 min in the phenobarbital group. Digitoxigenin disappeared at approximately the same rate from incubation flasks containing liver homogenates of phenobarbital-treated and control rabbits. Phenobarbital pretreatment had no influence on the formation of 3-ketodigitoxigenin (3-dehydrodigitoxigenin). Aniline hydroxylation, cytochrome P-450 content of liver microsomes, and liver weight were elevated in the phenobarbital-pretreated rabbits as compared to the controls. The milligrams of hepatic microsomal protein per g of liver were not significantly different between the two groups of animals.
Footnotes
- Received December 3, 1973.
- Copyright © 1974 by The American Society for Pharmacology and Experimental Therapeutics
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