Mass spectral analysis of an incubate of 2-NDHSA (substrate) with rat liver cytosols. A, HPLC-extracted ion chromatograms of m/z 276 for 2-NDHSA; B, extracted ion chromatograms of m/z 275 showing the formation of 2-NDASA; C, negative CID product ion spectrum of m/z 275; and D, positive CID product ion spectrum of m/z 277.
Effect of various enzyme inhibitors on the formation of 2-NDASA in rat liver cytosols. Results are expressed as means ± S.D. (n = 6). *, values that are significantly different (p < 0.05) from the corresponding results in the complete incubations.
Mass spectra of the 2-NDHSA incubation products in rat liver cytosol in the presence of either 14N-glutamic acid or 15N-glutamic acid. Positive full-scan MS spectra of 14N-2-NDASA (A) and a mixture of 14N-and 15N-2-NDASA (B). C, positive CID product ion spectrum of m/z 277 for 14N-2-NDASA; and D, CID product ion spectrum of m/z 278 for 15N-2-NDASA.