Abstract
The accurate estimation of “in vivo” inhibition constants (Ki) of inhibitors and fraction metabolized (fm) of substrates is highly important for drug–drug interaction (DDI) prediction based on physiologically based pharmacokinetic (PBPK) models. We hypothesized that analysis of the pharmacokinetic alterations of substrate metabolites in addition to the parent drug would enable accurate estimation of in vivo Ki and fm. Twenty-four pharmacokinetic DDIs caused by P450 inhibition were analyzed with PBPK models using an emerging parameter estimation method, the cluster Newton method, which enables efficient estimation of a large number of parameters to describe the pharmacokinetics of parent and metabolized drugs. For each DDI, two analyses were conducted (with or without substrate metabolite data), and the parameter estimates were compared with each other. In 17 out of 24 cases, inclusion of substrate metabolite information in PBPK analysis improved the reliability of both Ki and fm. Importantly, the estimated Ki for the same inhibitor from different DDI studies was generally consistent, suggesting that the estimated Ki from one study can be reliably used for the prediction of untested DDI cases with different victim drugs. Furthermore, a large discrepancy was observed between the reported in vitro Ki and the in vitro estimates for some inhibitors, and the current in vivo Ki estimates might be used as reference values when optimizing in vitro–in vivo extrapolation strategies. These results demonstrated that better use of substrate metabolite information in PBPK analysis of clinical DDI data can improve reliability of top-down parameter estimation and prediction of untested DDIs.
Footnotes
- Received April 3, 2018.
- Accepted August 16, 2018.
This work was supported by the Japan Society for the Promotion of Science (JSPS), Japan [a Grant-in-Aid for Scientific Research on Innovative Areas 22136001 and a Grant-in-Aid for Scientific Research (S) 24229002].
↵This article has supplemental material available at dmd.aspetjournals.org.
- Copyright © 2018 by The American Society for Pharmacology and Experimental Therapeutics