Fig. 2. (A) Relative metabolic activities of CYP2A, CYP2B, CYP3A, and CYP2C11 after systemic administration of oleamide, measured as the rate of testosterone hydroxylation and shown as the percentage of the rate of reaction in the control group. The absolute control values (picomoles per minute per milligram of total protein content) were as follows: 217.4 ± 23.7, 37.5 ± 6.1, 76.2 ± 14.8, 614.8 ± 92.0, 3303.0 ± 779.0, and 2934.5 ± 791.6 (testosterone 7α-, 16β-, 2β-, 6β-, 2α-, and 16α- hydroxylations, respectively). Animals were treated intraperitoneally with oleamide 0.1 (n = 10), 1 (n = 10), 10 mg/kg per day (n = 10), and propylene glycol (control group, n = 6). Reactions were performed in the presence of testosterone (400 µM) in RLMs (1 mg/ml of the total protein content) with an NADPH-generating system in a final volume of 1 ml at 37°C for 20 minutes. (B) Specific metabolic activities of CYP2A, CYP2B, CYP3A, and CYP2C11 after systemic administration of oleamide measured as the rate of testosterone hydroxylation per nanomoles of total P450 content per minute and shown as the percentage of the rate of reaction in the control group. The absolute control values (picomoles per minute per nanomoles of total P450 content) were as follows: 350.8 ± 38.8, 60.3 ± 8.2, 122.5 ± 20.2, 989.5 ± 127.0, 5321.8 ± 1188.7, and 4726.4 ± 1209.4 (testosterone 7α-, 16β-, 2β-, 6β-, 2α-, and 16α- hydroxylations, respectively). All values are expressed as bar graphs of the mean (S.D.). Statistical significance was assessed by the Kruskal-Wallis test, followed by multiple comparisons of mean ranks (A) and one-way ANOVA test (B). Statistical significance with respect to the control group (propylene glycol, n = 6) is indicated with **P ≤ 0.01.