Article Figures & Data
Figures
Tables
Data Supplement
- Supplemental Data -
Supplemental Material
Supplementary Figure 1 - CYP3A4 mRNA gene expression data from >50 individual repeated experiments conducted in a single laboratory with hepatocyte donor H4 as measured by real time PCR (TaqMan® RT-PCR)
Supplementary Figure 2 - CYP3A4 mRNA gene expression data from 70 individual repeated experiments conducted in a single laboratory with hepatocyte donor H12 as measured by real time PCR (TaqMan® RT-PCR)
Supplementary Figure 3 - Intra-donor basal and induced CYP3A enzyme activities and corresponding fold induction values across multiple repeated experiments in hepatocyte donors H2, H4 and H12
Supplementary Figure 4 - Untransformed Emax data for percent positive control dataset (Figure 5, panel A)
Supplementary Figure 5 - RIS and slope corrections
Supplementary Table 1 - Summary of methods used to generate single concentration flumazenil and rifampicin data in Figure 1 and Tables 2 and 3
Supplementary Table 2 - Review of the metabolic pathways for probe substrates in clinical studies for compounds for which DDI magnitude was probe dependent
Supplementary Table 3 - CYP3A4 mRNA EC50 and Emax values for rifampicin from six different sources collated via the IQ IWG and from literature
Supplementary Table 4 - CYP3A4 mRNA EC50 and Emax values for rifampicin in nine human hepatocyte donors following multiple repeats within a laboratory using the same experimental methodology gathered from three companies by the IQ IWG
Supplementary Table 5 - Prototypical inducer CYP3A4 mRNA EC50 and Emax values collated via the IQ IWG and from literature
Supplementary Table 6 - Summary of CYP3A in vitro induction parameters collected from IWG members and/or literature and clinical induction data for victim drugs with CYP3A mediated metabolism for 16 proprietary compounds collected from IQ member companies and 34 prototypical inducers collected from the UWDIDB
Supplementary Table 7 - Classification of clinical induction and category changes based on median or worst case AUCR
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