Abstract
A number of compounds with structural similarities to SKF 525-A (adiphenine, benactyzine, propoxyphene, diphenhydramine, desipramine, and nortriptyline) are capable of forming metabolite-cytochome P-450 complexes absorbing maximally at 455 nm when metabolized by rat hepatic microsomes. The optimum substrate concentrations for the maximum rate of formation of 455-nm complexes in microsomes from untreated rats and from rats pretreated with phenobarbital were between 25-50 µM. With microsomes from phenobarbital-induced rats, the maximum rate of formation of 455-nm complexes was proportional to the concentration of induced cytochrome P-450. The rate of formation of 455-nm complexes was highest with microsomes from phenobarbital-induced animals, intermediate with microsomes from untreated rats, and lowest with microsomes from 3-methylcholanthrene-inducd rats.
Footnotes
- Received February 4, 1974.
- Copyright © 1974 by The American Society for Pharmacology and Experimental Therapeutics
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