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- Data Supplement - Supplemental Fig.1. C. rodentium bacteria in the colons and livers of infected mice. Mice were infected p.o. with C. rodentium and bacteria from (A) colon and (B) liver samples were cultured on MacConkey agar post mortem. Data are expressed as bacterial cells per gram of tissue. Values represent means � S.E.M. of 6 mice per group except for the 15 day pairfed group (5 mice).
- Data Supplement - Supplemental Fig. 2. Histopathological analysis of colons of mice infected with C. rodentium. Representative colonic sections from mice treated as indicated on the figure were stained with hematoxylin and eosin, and viewed under 200X magnification.
- Data Supplement - Supplemental Fig. 3. Histopathological analysis of livers of mice infected with C. rodentium. H & E stained liver sections are as follows: A, Pairfed mouse, 7 days, 20 X; B, Infected mouse, 7 days, 20 X; C, Infected mouse, 10 days, 20 X; D, Infected mouse, 7 days, 40 X.
- Data Supplement - Supplemental Fig. 4. Effect of oral C. rodentium infection on food consumption and body weights. Food intake and body weights were measured daily. Consumption per cage was divided by 6 to obtain the average consumption per mouse in that group. Thus, the n values for food consumption from days 1- 7, 8-10. 11-15 and 16-24 were 4, 3, 2 and 1 cages respectively, and S.E.M. values could only be obtained for the first 10 days. The corresponding n values for body weights were 24, 18, 12 and 6 mice, respectively. * P <_0.05 compared="compared" to="to" untreated="untreated" _="_" p0.05="p0.05" pairfed.="pairfed." differences="differences" among="among" groups="groups" were="were" determined="determined" by="by" oneway="oneway" anova="anova" followed="followed" tukeys="tukeys" test.="test." _--="_--" end="end" desc="desc" dmd24240_supp_fig_4.tif="dmd24240_supp_fig_4.tif">
- Data Supplement - Supplemental Fig. 5. Body weights and colonic C. rodentium counts following parenteral injection of C. rodentium in mice. Mice were injected i.p. with the indicated number of C. rodentium cells, and killed 24 h later. A. Mouse body weights before and after infection. B. Bacteria from colon samples were cultured on McConkey agar post mortem. Data are expressed as bacterial cells per g of tissue. Values represent means � S.E.M. of 6 mice per group. . * P <_0.05 compared="compared" to="to" control.="control." differences="differences" between="between" treated="treated" groups="groups" and="and" control="control" were="were" determined="determined" by="by" oneway="oneway" anova="anova" followed="followed" dunnetts="dunnetts" test--="test--" end="end" desc="desc" dmd24240_supp_fig_5.tif="dmd24240_supp_fig_5.tif" _--="_--">
- Data Supplement - Supplemental Fig. 6 Serum cytokine profile following parenteral C. rodentium infection in mice. Mice were injected i.p. with the indicated number of C. rodentium cells, and killed 24 h later. Blood was collected at sacrifice for measurement of serum cytokines as described in Materials and Methods. Values represent means � S.E.M. of 6 mice per group. * P <_0.05 compared="compared" to="to" saline-injected="saline-injected" control="control" group.="group." differences="differences" between="between" treated="treated" groups="groups" and="and" were="were" determined="determined" by="by" oneway="oneway" anova="anova" followed="followed" dunnetts="dunnetts" test.--="test.--" end="end" desc="desc" dmd24240_supp_fig_6.tif="dmd24240_supp_fig_6.tif" _--="_--">
- Data Supplement - Supplemental Fig. 7 Effects of parenteral infection of mice with C. rodentium on hepatic microsomal P450 proteins. Mice were injected i.p. with 108 C. rodentium cells, and killed 12 h later. Livers were harvested for measurement of microsomal P450 protein levels as described in Materials and Methods. Values represent means � S.E.M. of 6 mice per group, and are calculated relative to the mean of the saline-injected control group. * P <_0.05 compared="compared" to="to" control="control" students="students" t-test.="t-test." _--="_--" end="end" desc="desc" dmd24240_supp_fig_7.tif="dmd24240_supp_fig_7.tif">
- Data Supplement
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Supplemental Table 1. Colon and liver weights of infected and control mice.
Supplemental Table 2 Time course of colonic pathology in infected and control mice.
Supplemental Table 3. Liver inflammation in C. rodentium-infected mice.