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- Data Supplement - Supplemental Figure 1. Effects of TNFα and retinoid receptor agonists on the CYP4F11 promoter activity. The effect of TNFα and retinoid receptor agonists were tested in HaCaT cells using a luciferase assay. Promoter fragments were generated by PCR as described under materials and methods, ligated and cloned upstream of the promoterless luciferase reporter gene ** vector as indicated. Twenty-four hours after transfection, cells were treated with or without retinoid agonists (all at the concentration of 1μM) for 2 days and then treated with 10 ng/ml TNFα for 24 hours. Data are presented as the mean luciferase activity adjusted for β-gal activity (�SEM) and compared with control wells of three independent transfections. Each data point represents n=3. *P< 0.05, **P< 0.01 compared with the control group.