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Research ArticleArticle

Optimization of Experimental Conditions of Automated Glucuronidation Assays in Human Liver Microsomes using a Cocktail Approach and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Justine Badee, Nahong Qiu, Neil John Parrott, Abby C. Collier, Stephan Schmidt and Stephen Fowler
Drug Metabolism and Disposition November 26, 2018, dmd.118.084301; DOI: https://doi.org/10.1124/dmd.118.084301
Justine Badee
University of Florida;
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  • For correspondence: stephen.fowler@roche.com jbadee@cop.ufl.edu
Nahong Qiu
F.Hoffmann-La Roche Ltd;
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Neil John Parrott
F.Hoffmann-La Roche Ltd;
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Abby C. Collier
University of British Columbia
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Stephan Schmidt
University of Florida;
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Stephen Fowler
F.Hoffmann-La Roche Ltd;
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  • For correspondence: stephen.fowler@roche.com jbadee@cop.ufl.edu
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      Supplemental Figure 1 - Metabolism of UGT probe substrates used for assessing glucuronidation activities in human liver microsomes

      Supplemental Table 1 - Published Km values (μM) for 10 UGT probe substrates in the absence and presence of 2% BSA (w/v) in human liver microsomes and recombinantly expressed human UGT enzyme preparations

      References

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Drug Metabolism and Disposition: 48 (1)
Drug Metabolism and Disposition
Vol. 48, Issue 1
1 Jan 2020
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Research ArticleArticle

Optimization of Experimental Conditions of Automated Glucuronidation Assays in Human Liver Microsomes using a Cocktail Approach and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Justine Badee, Nahong Qiu, Neil John Parrott, Abby C. Collier, Stephan Schmidt and Stephen Fowler
Drug Metabolism and Disposition November 26, 2018, dmd.118.084301; DOI: https://doi.org/10.1124/dmd.118.084301

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Research ArticleArticle

Optimization of Experimental Conditions of Automated Glucuronidation Assays in Human Liver Microsomes using a Cocktail Approach and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Justine Badee, Nahong Qiu, Neil John Parrott, Abby C. Collier, Stephan Schmidt and Stephen Fowler
Drug Metabolism and Disposition November 26, 2018, dmd.118.084301; DOI: https://doi.org/10.1124/dmd.118.084301
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