Data Supplement
- Supplemental Data -
Supplemental Methods
Supplemental Table S1 - Target peptide sequences and selected/multiple reaction monitoring (SRM/MRM) transitions for quantification of each protein
Supplemental Table S2 - Molecules under the limit of quantification
Supplemental Table S3 - Summery of the zonated expressions of transporters and metabolizing enzymes in peri-portal vein (PP) or peri-central vein (PC) regions of the liver in rodents
References
Supplemental Figure S1 - Uneven distribution of SR-101 in the liver acinus of mice at 30 min (A) or 90 min (B and C) after intravenous injection of SR-101
Supplemental Figure S2 - A comparison of protein expression levels of transporters/receptors (A) and metabolizing enzymes (B) in the SR-101(+) and SR-101(-) liver regions of mice intravenously injected with SR-101
Supplemental Figure S3 - Mouse Oatp2b1 mediates SR-101 uptake. (A) Representative fluorescence images of SR-101 in control HEK293 cells (Control/HEK293; A1 and A2) and mouse Oatp2b1-overexpressing HEK293 cells (Oatp2b1/HEK293; A3 and A4)
Supplemental Figure S4A - comparison of protein expression levels of transporters, normalized by the level of 4F2hc, in the plasma membrane fractions of total mouse liver and laser-microdissected samples from frozen mouse liver sections.