TY - JOUR T1 - Species Independence in Brain Tissue Binding Using Brain Homogenates JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1270 LP - 1277 DO - 10.1124/dmd.111.038778 VL - 39 IS - 7 AU - Li Di AU - John P. Umland AU - George Chang AU - Youping Huang AU - Zhen Lin AU - Dennis O. Scott AU - Matthew D. Troutman AU - Theodore E. Liston Y1 - 2011/07/01 UR - http://dmd.aspetjournals.org/content/39/7/1270.abstract N2 - Species independence of brain tissue binding was assessed with a large number of structurally diverse compounds using equilibrium dialysis with brain homogenates of seven species and strains (Wistar Han rat, Sprague-Dawley rat, CD-1 mouse, Hartley guinea pig, beagle dog, cynomolgus monkey, and human). The results showed that the fractions unbound of the seven species and strains were strongly correlated with correlation coefficients ranging from 0.93 to 0.99. The cross-species/strain correlations were not significantly different from the interassay correlation with the same species. The linear correlation between Wistar Han and other species had a slope close to 1 and an intercept near 0. Based on orthogonal statistical analysis, no correction is needed for extrapolation of fraction unbound from Wistar Han rat to the other species or strains. Hence, brain tissue binding of Wistar Han rat can be used to obtain binding of other species and strains in drug discovery. ER -