RT Journal Article SR Electronic T1 125I-insulin metabolism by rat liver cells: preferential binding and degradation of insulin labeled at TyrA14. JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 153 OP 155 VO 10 IS 2 A1 R I Misbin A1 S J Buynitzky A1 E C Almira YR 1982 UL http://dmd.aspetjournals.org/content/10/2/153.abstract AB The binding and degradation of highly purified A14-[125I]monoiodoinsulin and A19-[125I]monoiodoinsulin were compared in isolated rat hepatocytes. A14-125I-insulin was bound more rapidly and was degraded more rapidly than was A19-125I-insulin. The difference in the rate of degradation between the two preparations was apparent when either tricholoroacetic acid precipitation or binding to specific insulin receptors on cultured human lymphocytes was used for assay. These results provide evidence that binding to its receptor is the first step in insulin degradation by liver cells.