PT  - JOURNAL ARTICLE
AU  - Goromaru, T
AU  - Matsuura, H
AU  - Furuta, T
AU  - Baba, S
AU  - Yoshimura, N
AU  - Miyawaki, T
AU  - Sameshima, T
TI  - Identification of fentanyl metabolites in rat urine by gas chromatography-mass spectrometry with stable-isotope tracers.
DP  - 1982 Sep 01
TA  - Drug Metabolism and Disposition
PG  - 542--546
VI  - 10
IP  - 5
4099  - http://dmd.aspetjournals.org/content/10/5/542.short
4100  - http://dmd.aspetjournals.org/content/10/5/542.full
SO  - Drug Metab Dispos1982 Sep 01; 10
AB  - The metabolites of fentanyl (l), which has been widely used as a neuroleptic analgesic agent, were identified in urine of rats by gas chromatography-mass spectrometry combined with a stable-isotope tracer technique. After the oral administration of an equimolar mixture of l and deuterium-labeled l (l/l-d5), the urinary metabolites were extracted with chloroform at pH 9.0. Extracts were derivatized and analyzed by GC/MS. Metabolites were identified by the presence of doublet ion peaks separated by 5 amu, and chemical structures were established from analyses of fragmentation pathways. The metabolites were identified as 4-N-(N-propionylanilino)-piperidine, 4-N-(N-hydroxypropionylanilino)piperidine, 4-N-(N-propionylanilino) hydroxypiperidine, 1-(2-phenethyl)-4-N-(N-hydroxypropionylanilino)piperidine and 1-(2-phenethyl)-4-N-(N-propionylanilino)hydroxypiperidine. These metabolites, together with unchanged l, were also detected in urine of rats receiving l/l-d5 intravenously, by selected-ion monitoring of the specific cluster ions.