TY - JOUR T1 - A new pyrrolizidine alkaloid metabolite, 19-hydroxysenecionine isolated from mouse hepatic microsomes in vitro. JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 696 LP - 699 VL - 10 IS - 6 AU - D F Eastman AU - H J Segall Y1 - 1982/11/01 UR - http://dmd.aspetjournals.org/content/10/6/696.abstract N2 - The in vitro mouse hepatic microsomal metabolism of the macrocyclic pyrrolizidine alkaloid senecionine was studied by high-performance liquid chromatography. A muBondapak-C18 reverse-phase system was developed to study the senecionine metabolites over a wide range of polarities. Methods were further developed for the isolation of each individual metabolite. Senecic acid, senecionine N-oxide, and 19-hydroxysenecionine, a new metabolite, were isolated from the microsomal enzyme system of BALB/c mice. The metabolite, dehydroretronecine, which had previously been isolated from rat hepatic microsomes, was not detected, and minor metabolites were not identified. ER -