PT  - JOURNAL ARTICLE
AU  - RICHARD M. THOMPSON
AU  - NICHOLAS GERBER
AU  - RICHARD A. SEIBERT
AU  - DOMINIC M. DESIDERIO
TI  - A RAPID METHOD FOR THE MASS SPECTROMETRIC IDENTIFICATION OF GLUCURONIDES AND OTHER POLAR DRUG METABOLITES IN PERMETHYLATED RAT BILE
DP  - 1973 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 489--505
VI  - 1
IP  - 2
4099  - http://dmd.aspetjournals.org/content/1/2/489.short
4100  - http://dmd.aspetjournals.org/content/1/2/489.full
SO  - Drug Metab Dispos1973 Mar 01; 1
AB  - A technique for rapidly screening drugs and other compounds for possible glucuronide formation has been developed. The drug is either added to the perfusate of an isolated perfused rat liver or injected directly into the portal vein of an anesthetized rat. Bile is collected, dried, and permethylated with dimethyl sulfoxide sodium salt and methyl iodide. The product mixture is analyzed by mass spectrometry through both the direct and gas chromatography inlets. Glucuronides are identified by the presence of major ions at m/e 101 and m/e 201. Intact glucuronides of hydroxydiphenylhydantoin, hydroxymephobarbital (or hydroxyphenobarbital), hydroxyhexobarbital, chloramphenicol, 2-methyl-1,4-dihydroxynaphthalene (reduced menadione), and others have been detected. The technique can be conveniently applied to 1-5 µl of bile (estimated 0.1-1 µg of drug or each metabolite). Other metabolites can also be identified. Copyright © 1973 by The American Society for Pharmacology and Experimental Therapeutics