@article {SCOTT506, author = {K. N. SCOTT and M. W. COUCH and B. J. WILDER and C. M. WILLIAMS}, title = {THE USE OF MASS SPECTROSCOPY AND 13C AND 1H NUCLEAR MAGNETIC RESONANCE IN THE CHARACTERIZATION OF 1-METHYL-(2,6-XYLYLOXY)ETHYLAMINE METABOLITES}, volume = {1}, number = {2}, pages = {506--515}, year = {1973}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The metabolism of a recently developed anticonvulsant drug, 1-methyl-(2,6-xylyloxy)ethylamine (K{\"O} 1173), was investigated by a combination of gas chromatography, chemical ionization and high-resolution electron impact mass spectrometry, and proton and 13C nuclear magnetic resonance spectrometry. Two unknown metabolites in the urine of a patient taking the drug were trapped in milligram amounts by preparative gas chromatography. In conjunction with chemical ionization, high-resolution electron impact mass spectrometry gave unequivocal empirical formulae for the two peaks: each metabolite had the same empirical formula as the drug plus one oxygen atom. Proton and 13C nuclear magnetic resonance spectrometry showed that one metabolite was the free base having a hydroxyl group para to the ether linkage, and the other metabolite was the free base having one of the methyl groups replaced by a CH2OH group. The four spectroscopic techniques employed provide a powerful tool for the identification of drug metabolites. Copyright {\textcopyright} 1973 by The American Society for Pharmacology and Experimental Therapeutics}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/1/2/506}, eprint = {https://dmd.aspetjournals.org/content/1/2/506.full.pdf}, journal = {Drug Metabolism and Disposition} }