RT Journal Article SR Electronic T1 The effect of pregnancy and treatment with 17 beta-estradiol on the transport of organic anions into isolated rat hepatocytes. JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 713 OP 716 VO 12 IS 6 A1 W J Brock A1 M Vore YR 1984 UL http://dmd.aspetjournals.org/content/12/6/713.abstract AB The uptake of the bile acid taurocholate (TC), and the organic anions, estradiol-17 beta (beta-D-glucuronide) (E217G), estradiol-3-(beta-D-glucuronide) (E23G), estriol-16 alpha (beta-D-glucuronide) (E316G), and morphine glucuronide (MG) were evaluated in hepatocytes isolated from nonpregnant female, pregnant (19-21 days of gestation) and E2-treated (1 mg/kg/day sc for 14 days) rats. Pregnancy significantly decreased the uptake of TC, E217G, E23G, and MG whereas E2 treatment decreased only the uptake of E217G. The Vmax (nmol/min/mg protein) for E217G uptake was significantly decreased from 1.45 +/- 0.2 (mean +/- SE) in hepatocytes from nonpregnant female rats to 0.70 +/- 0.11 and 0.64 +/- 0.13 in cells from pregnant and E2-treated rats, respectively. The Vmax for uptake of TC was decreased, but not significantly, from 0.56 +/- 0.16 in hepatocytes from nonpregnant female rats to 0.34 +/- 0.08 in cells from pregnant rats.