PT  - JOURNAL ARTICLE
AU  - S Meloche
AU  - J G Besner
TI  - Metabolism of isotretinoin. Biliary excretion of isotretinoin glucuronide in the rat.
DP  - 1986 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 246--249
VI  - 14
IP  - 2
4099  - http://dmd.aspetjournals.org/content/14/2/246.short
4100  - http://dmd.aspetjournals.org/content/14/2/246.full
SO  - Drug Metab Dispos1986 Mar 01; 14
AB  - The biliary metabolites of isotretinoin were examined after iv administration of 4-20-mg/kg doses to vitamin A-normal bile duct-cannulated rats. Analysis of bile by reverse phase high performance liquid chromatography showed that injection of isotretinoin is followed by a rapid excretion of metabolites in bile. Isotretinoin glucuronide was identified as the major metabolite in bile. A specific high performance liquid chromatography method based on the assay of generated isotretinoin in beta-glucuronidase-treated bile was developed for the determination of isotretinoin glucuronide in bile samples. The excretion rate of isotretinoin glucuronide increased rapidly to reach a maximum 55 min after dosing and then declined exponentially. After 330 min of collection, biliary excretion of isotretinoin glucuronide was almost complete, and the metabolite accounted for 34.8-37.9% of the dose. These results indicate that conjugation with glucuronic acid represents a major pathway for the metabolism of pharmacological doses of isotretinoin. The maximum excretion rate of isotretinoin glucuronide in bile increased in a linear manner with the dose of isotretinoin, and no delay was observed after the larger doses. These data suggest that glucuronidation and biliary excretion are not saturated at high pharmacological doses of isotretinoin.