RT Journal Article
SR Electronic
T1 Phase II biotransformation of carcinogens/atherogens in cultured aortic tissues and cells. II. Glucuronidation of 3-hydroxy-benzo(a)pyrene.
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 293
OP 298
VO 14
IS 3
A1 H Y Yang
A1 M W Majesky
A1 M J Namkung
A1 M R Juchau
YR 1986
UL http://dmd.aspetjournals.org/content/14/3/293.abstract
AB Avian aortic tissues contain active UDP-glucuronosyltransferase(s) (EC 2.4.1.17) which catalyze(s) the glucuronidation of 3-hydroxybenzo(a)pyrene and p-nitrophenol. Activities were compared in abdominal segments (susceptible to the atheroma-promoting effects of polynuclear aromatic hydrocarbons) vs. thoracic segments (susceptible to the atheroma-initiating effects of polynuclear aromatic hydrocarbons). Activities measured in the abdominal segments were approximately 8-9-fold higher than those measured in thoracic segments from the same cockerels. Surprisingly, pretreatment of cockerels with phenobarbital, but not 3-methylcholanthrene, resulted in small (60-120%) but consistent increases in glucuronosyltransferase activities in both aortic segments. Activities were readily detectable in microsomal fractions of both segments and in cultured smooth muscle cells derived from aortic segments, but were not detectable in cultured endothelial cells. Avian aortic microsomal glucuronosyltransferases exhibited minimal or no response to the effects of several common activators of hepatic microsomal glucuronosyltransferases.