TY - JOUR T1 - Glutathione conjugates. Immobilized enzyme synthesis and characterization by fast atom bombardment mass spectrometry. JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 313 LP - 318 VL - 14 IS - 3 AU - S L Pallante AU - C A Lisek AU - D M Dulik AU - C Fenselau Y1 - 1986/05/01 UR - http://dmd.aspetjournals.org/content/14/3/313.abstract N2 - Glutathione transferase activity was shown to be present in an immobilized preparation of microsomal protein. Chlorodinitrobenzene, ethacrynic acid, captopril, styrene oxide, and iminocyclophosphamide were found to be substrates, each providing a different kind of electrophilic functional group for conjugation. The glutathione conjugates were characterized by thin layer chromatography (visualized by reaction with ninhydrin) and by high pressure liquid chromatography. A variety of conditions was evaluated for analysis of these glutathiones by fast atom bombardment mass spectrometry. ER -