@article {Ishida77, author = {T Ishida and Y Kumagai and Y Ikeda and K Ito and M Yano and S Toki and K Mihashi and T Fujioka and Y Iwase and S Hachiyama}, title = {(8S)-(glutathion-S-yl)dihydromorphinone, a novel metabolite of morphine from guinea pig bile.}, volume = {17}, number = {1}, pages = {77--81}, year = {1989}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {A novel glutathione-conjugated metabolite of morphine has been isolated from the bile of guinea pigs given morphine. The metabolite was separated by preparative HPLC on a reverse phase column (YMC-GEL C18) using methanol/water (1:4, v/v) as eluate and purified by HPLC on another reverse phase column (mu-Bondapak phenyl) using water/acetonitrile/trimethylamine/acetic acid (150:3:2:1, v/v) as a mobile phase. The unambiguous structure assignment of the metabolite was performed by fast atom bombardment mass spectrometry and 400 MHz fourier transform NMR spectrometric analysis, and it was identified as (8S)-glutathion-S-yl)dihydromorphinone, in comparison with the synthetic morphinone-glutathione adduct.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/17/1/77}, eprint = {https://dmd.aspetjournals.org/content/17/1/77.full.pdf}, journal = {Drug Metabolism and Disposition} }