PT  - JOURNAL ARTICLE
AU  - T Inaba
AU  - A Tait
AU  - M Nakano
AU  - W A Mahon
AU  - W Kalow
TI  - Metabolism of diazepam in vitro by human liver. Independent variability of N-demethylation and C3-hydroxylation.
DP  - 1988 Jul 01
TA  - Drug Metabolism and Disposition
PG  - 605--608
VI  - 16
IP  - 4
4099  - http://dmd.aspetjournals.org/content/16/4/605.short
4100  - http://dmd.aspetjournals.org/content/16/4/605.full
SO  - Drug Metab Dispos1988 Jul 01; 16
AB  - The interindividual variability of diazepam metabolism was studied using human livers. The formation of N-desmethyldiazepam (NDZ) and 3-hydroxydiazepam (temazepam, TMZ), was monitored by gas chromatography. In 10 livers, Km values for NDZ and TMZ formation varied independently from each other, each by a factor of 4, from 100 to 400 microM. This variability is consistent with the presence of at least two different cytochrome P-450 species controlling formation of these metabolites. In the same livers, Vmax for NDZ and TMZ production varied 6-fold and 15-fold, respectively.