TY - JOUR T1 - Metabolism of diazepam in vitro by human liver. Independent variability of N-demethylation and C3-hydroxylation. JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 605 LP - 608 VL - 16 IS - 4 AU - T Inaba AU - A Tait AU - M Nakano AU - W A Mahon AU - W Kalow Y1 - 1988/07/01 UR - http://dmd.aspetjournals.org/content/16/4/605.abstract N2 - The interindividual variability of diazepam metabolism was studied using human livers. The formation of N-desmethyldiazepam (NDZ) and 3-hydroxydiazepam (temazepam, TMZ), was monitored by gas chromatography. In 10 livers, Km values for NDZ and TMZ formation varied independently from each other, each by a factor of 4, from 100 to 400 microM. This variability is consistent with the presence of at least two different cytochrome P-450 species controlling formation of these metabolites. In the same livers, Vmax for NDZ and TMZ production varied 6-fold and 15-fold, respectively. ER -