@article {Knadler89, author = {M P Knadler and B L Ackermann and J E Coutant and G H Hurst}, title = {Metabolism of the anticoagulant peptide, MDL 28,050, in rats.}, volume = {20}, number = {1}, pages = {89--95}, year = {1992}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Rats were each administered a 9 mg/kg iv bolus dose of a 3H-labeled decapeptide anticoagulant, MDL 28,050. Tritium was eliminated rapidly with approximately 50\% of the dose recovered in urine within the first 6 hr. Renal excretion accounted for 68\% of the dose, whereas fecal excretion accounted for 16\% of the dose. Continuous flow fast atom bombardment mass spectrometry was used to identify the major urinary metabolites of MDL 28,050. Trace amounts of parent drug were found, and other biotransformation products indicated that hydrolysis had occurred at four peptide bonds. Two initial sites of hydrolysis were identified as 4I-5P and 6E-7E, which resulted in the peptide fragments Suc-Y-E-P-I-OH + P-E-E-A-Cha-E-OH and Suc-Y-E-P-I-P-E-OH + E-A-Cha-E-OH, respectively. Further metabolism of these fragments resulted in the N-terminal pentapeptide and the C-terminal dipeptide.}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/20/1/89}, eprint = {https://dmd.aspetjournals.org/content/20/1/89.full.pdf}, journal = {Drug Metabolism and Disposition} }