RT Journal Article SR Electronic T1 Microbial models of mammalian metabolism. Furosemide glucoside formation using the fungus Cunninghamella elegans. JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 259 OP 267 VO 21 IS 2 A1 M Hezari A1 P J Davis YR 1993 UL http://dmd.aspetjournals.org/content/21/2/259.abstract AB The diuretic furosemide (Lasix) was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to the phase II conjugate, furosemide acyl glucoside. This metabolite was isolated following semipreparative scale incubations of C. elegans involving glucose nutrient dosing, and was characterized by NMR spectroscopy (1H and 1H/1H correlated), MS (FAB), UV, HPLC with fluorescence detection, and enzymatic treatments. The aglycone fragment of the conjugate was characterized as furosemide by treatment of the metabolite with sodium hydroxide, whereas the sugar part was identified as glucose by cleavage of the conjugate, derivatization of the released sugar, and GC/MS analysis.