TY - JOUR T1 - Short-term maintenance of phase I and II metabolism in precision-cut liver slices in dynamic organ culture. JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 364 LP - 366 VL - 24 IS - 3 AU - S Ekins Y1 - 1996/03/01 UR - http://dmd.aspetjournals.org/content/24/3/364.abstract N2 - Testosterone and 7-ethoxycoumarin were used as substrates to quantify the maintenance of phase I and II enzymes in precision-cut rat liver slices in dynamic organ culture. Testosterone hydroxylations, 7-ethoxycoumarin O-deethylation, 7-hydroxycoumarin sulfate, and 7-hydroxycoumarin glucuronide formation were all maintained at initial levels in the slice incubation media after incubation for up to 4 hr. The activities of various cytochrome P450 isozymes, measured using the stereospecific and regiospecific hydroxylation of testosterone and the maintenance of phase I and II metabolism using 7-ethoxycoumarin, are therefore suggested to be stable over short-term incubations in a physiological buffer. The testosterone hydroxylation assay is also suggested as a versatile slice metabolic viability marker for various P450 activities over longer periods. ER -