TY - JOUR T1 - THE <em>IN VITRO</em> HYDROLYSIS OF DI-(2-ETHYLHEXYL) PHTHALATE BY RAT TISSUES JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 341 LP - 344 VL - 2 IS - 4 AU - JAMES E. CARTER AU - DAVID B. ROLL AU - ROBERT V. PETERSEN Y1 - 1974/07/01 UR - http://dmd.aspetjournals.org/content/2/4/341.abstract N2 - The in vitro hydrolysis of di-(2-ethylhexyl) phthalate to mono-(2-ethylhexyl) phthalate and 2-ethylhexanol is reported. The metabolites have been identified by gas chromatography with supporting experiments employing 14C- and tritium-labeled substrate. The monoester, methylated to form methyl (2-ethylhexyl) phthalate, and 2-ethylhexanol were detected. An esterase or esterases present in rat liver, lung, and kidney appear(s) responsible for the hydrolysis. Subcellular fractionation of the liver indicates that hydrolysis takes place in mitochondria and microsomes but not in cytosol. Copyright © 1974 by The American Society for Pharmacology and Experimental Therapeutics ER -