PT  - JOURNAL ARTICLE
AU  - W. H. BULGER
AU  - S. J. STOHS
TI  - THE EFFECT OF PHENOBARBITAL PRETREATMENT ON THE
BIOTRANSFORMATION OF <sup>3</sup>H-DIGITOXIGENIN TO
6β-HYDROXY-3-EPIDIGITOXIGENIN BY RABBIT LIVER
HOMOGENATES
DP  - 1974 Jul 01
TA  - Drug Metabolism and Disposition
PG  - 361--364
VI  - 2
IP  - 4
4099  - http://dmd.aspetjournals.org/content/2/4/361.short
4100  - http://dmd.aspetjournals.org/content/2/4/361.full
SO  - Drug Metab Dispos1974 Jul 01; 2
AB  - 3H-Digitoxigenin was incubated over a time course of 15, 30, 45, and 60 min with liver homogenates prepared from phenobarbital-pretreated and control male rabbits. Pretreatment with phenobarbital preferentially enhanced the formation of 6β-hydroxy-3-epidigitoxigenin at all time points and significantly decreased the accumulation of 3-epidigitoxigenin at 45 and 60 min. The formation of 5β-hydroxydigitoxigenin was significantly greater at 15, 30, and 45 min in the phenobarbital group. Digitoxigenin disappeared at approximately the same rate from incubation flasks containing liver homogenates of phenobarbital-treated and control rabbits. Phenobarbital pretreatment had no influence on the formation of 3-ketodigitoxigenin (3-dehydrodigitoxigenin). Aniline hydroxylation, cytochrome P-450 content of liver microsomes, and liver weight were elevated in the phenobarbital-pretreated rabbits as compared to the controls. The milligrams of hepatic microsomal protein per g of liver were not significantly different between the two groups of animals. Copyright © 1974 by The American Society for Pharmacology and Experimental Therapeutics