%0 Journal Article %A F. Peter Guengerich %A Asit Parikh %A Eric F. Johnson %A Toby H. Richardson %A Claes von Wachenfeldt %A Jose Cosme %A Frank Jung %A Christian P. Strassburg %A Michael P. Manns %A Robert H. Tukey %A Mike Pritchard %A Sylvie Fournel-Gigleux %A Brian Burchell %T Heterologous Expression of Human Drug-metabolizing Enzymes %D 1997 %J Drug Metabolism and Disposition %P 1234-1241 %V 25 %N 11 %X This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient and stable mammalian cells. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enzyme products, antibody production, and production of proteins for biophysical studies. The American Society for Pharmacology and Experimental Therapeutics %U https://dmd.aspetjournals.org/content/dmd/25/11/1234.full.pdf