TY - JOUR T1 - Heterologous Expression of Human Drug-metabolizing Enzymes JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1234 LP - 1241 VL - 25 IS - 11 AU - F. Peter Guengerich AU - Asit Parikh AU - Eric F. Johnson AU - Toby H. Richardson AU - Claes von Wachenfeldt AU - Jose Cosme AU - Frank Jung AU - Christian P. Strassburg AU - Michael P. Manns AU - Robert H. Tukey AU - Mike Pritchard AU - Sylvie Fournel-Gigleux AU - Brian Burchell Y1 - 1997/11/01 UR - http://dmd.aspetjournals.org/content/25/11/1234.abstract N2 - This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient and stable mammalian cells. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enzyme products, antibody production, and production of proteins for biophysical studies. The American Society for Pharmacology and Experimental Therapeutics ER -