RT Journal Article
SR Electronic
T1 Heterologous Expression of Human Drug-metabolizing Enzymes
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 1234
OP 1241
VO 25
IS 11
A1 Guengerich, F. Peter
A1 Parikh, Asit
A1 Johnson, Eric F.
A1 Richardson, Toby H.
A1 von Wachenfeldt, Claes
A1 Cosme, Jose
A1 Jung, Frank
A1 Strassburg, Christian P.
A1 Manns, Michael P.
A1 Tukey, Robert H.
A1 Pritchard, Mike
A1 Fournel-Gigleux, Sylvie
A1 Burchell, Brian
YR 1997
UL http://dmd.aspetjournals.org/content/25/11/1234.abstract
AB This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient and stable mammalian cells. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enzyme products, antibody production, and production of proteins for biophysical studies. The American Society for Pharmacology and Experimental Therapeutics