PT  - JOURNAL ARTICLE
AU  - Olga V. Trubetskoy
AU  - Peter M. Shaw
TI  - A Fluorescent Assay Amenable to Measuring Production of β-<span class="sc">d</span>-Glucuronides  Produced from Recombinant UDP-Glycosyl Transferase Enzymes
DP  - 1999 May 01
TA  - Drug Metabolism and Disposition
PG  - 555--557
VI  - 27
IP  - 5
4099  - http://dmd.aspetjournals.org/content/27/5/555.short
4100  - http://dmd.aspetjournals.org/content/27/5/555.full
SO  - Drug Metab Dispos1999 May 01; 27
AB  - β-glucuronidase cleavage of 4-methylumbelliferyl β-d-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other β-d-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl β-d-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of β-D glucuronides generated using recombinant glycosyl transferase preparations. The American Society for Pharmacology and Experimental Therapeutics