RT Journal Article
SR Electronic
T1 A Fluorescent Assay Amenable to Measuring Production of β-<span class="sc">d</span>-Glucuronides  Produced from Recombinant UDP-Glycosyl Transferase Enzymes
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 555
OP 557
VO 27
IS 5
A1 Trubetskoy, Olga V.
A1 Shaw, Peter M.
YR 1999
UL http://dmd.aspetjournals.org/content/27/5/555.abstract
AB β-glucuronidase cleavage of 4-methylumbelliferyl β-d-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other β-d-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl β-d-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of β-D glucuronides generated using recombinant glycosyl transferase preparations. The American Society for Pharmacology and Experimental Therapeutics