RT Journal Article SR Electronic T1 Detection of Cytochrome P450 and Other Drug-Metabolizing Enzyme mRNAs in Peripheral Blood Mononuclear Cells Using DNA Arrays JF Drug Metabolism and Disposition JO Drug Metab Dispos FD American Society for Pharmacology and Experimental Therapeutics SP 987 OP 993 VO 28 IS 8 A1 Linh T. Nguyen A1 Murali Ramanathan A1 Bianca Weinstock-Guttman A1 Kiran Dole A1 Colleen Miller A1 Margaret Planter A1 Kara Patrick A1 Carol Brownscheidle A1 Lawrence D. Jacobs YR 2000 UL http://dmd.aspetjournals.org/content/28/8/987.abstract AB The purpose of this study was to determine whether the expression of cytochrome P450 (CYP) enzyme mRNAs, other drug-metabolizing enzyme mRNAs, and transporter mRNAs can be detected using DNA arrays. Total RNA was isolated from peripheral blood mononuclear cells of 10 multiple sclerosis patients and 10 age- and sex-matched controls. The mRNA was reverse transcribed to radiolabeled cDNA, and the resultant cDNA was used to probe a DNA array containing several thousand known human genes. The signals corresponding to several CYPs, drug-metabolizing, and transporter mRNAs was substantially above background. The results demonstrate that the DNA array technique has the sensitivity and the selectivity for applications in the pharmaceutical sciences. The mean values for mRNAs of specific CYPs and drug-metabolizing enzymes in peripheral blood cells were compared with reported values for liver. The capabilities of DNA arrays may prove useful for characterizing CYP expression in a variety of clinical samples. The American Society for Pharmacology and Experimental Therapeutics