RT Journal Article
SR Electronic
T1 Identification of a New Human <em>CYP2A</em> Gene Fragment with Close Linkage to <em>CYP2GP1</em>
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 4
OP 7
VO 29
IS 1
A1 Jiangjun Sheng
A1 Zichun Hua
A1 Jiancheng Guo
A1 Michele Caggana
A1 Xinxin Ding
YR 2001
UL http://dmd.aspetjournals.org/content/29/1/4.abstract
AB Human genomic libraries were screened to identifyCYP2G-related cytochrome P450 genes. A genomic fragment comprising exons 7 through 9 of CYP2GP1 and exons 6 through 9 of a previously unidentified CYP2A gene, designated CYP2A7P2, was isolated from an EMBL3 library; the two genes were arranged in outward opposite directions with about 8 kbp of intervening sequence. The same structure was also detected in a bacteriophage P1 clone, which contained a full-lengthCYP2GP1 gene, exons 6 through 9 ofCYP2A7P2, and the CYP2B7 gene. However, additional CYP2A-related exons as well as otherCYP2A genes, CYP2A7P1,CYP2B6, CYP2F1, andCYP2GP2 were not detected. These results indicate thatCYP2A7P2 is located near CYP2B7 in the middle of the CYP2A-2B-2F gene cluster on chromosome 19. Furthermore, an analysis of CYP2A sequence alignment suggests thatCYP2A7P2 may be derived from the same ancestral gene that gave rise to CYP2A7P1, which was corrupted by a large insertion at intron 5. The American Society for Pharmacology and Experimental Therapeutics