TY - JOUR T1 - Application of Directly Coupled High Performance Liquid Chromatography-NMR-Mass Spectometry and <sup>1</sup>H NMR Spectroscopic Studies to the Investigation of 2,3-Benzofuran Metabolism in Sprague-Dawley Rats JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1357 LP - 1363 DO - 10.1124/dmd.30.12.1357 VL - 30 IS - 12 AU - John C. Connelly AU - Susan C. Connor AU - Soria Monte AU - Nigel J.C. Bailey AU - Nathan Borgeaud AU - Elaine Holmes AU - Jeff Troke AU - Jeremy K. Nicholson AU - Claire L. Gavaghan Y1 - 2002/12/01 UR - http://dmd.aspetjournals.org/content/30/12/1357.abstract N2 - The urinary excretion of metabolites of 2,3-benzofuran was studied in Sprague-Dawley rats (n = 5) given a single dose of 150 mg/kg i.p. Urine samples were collected at defined intervals up to 7 days postdose and analyzed using 1H NMR and directly coupled high performance liquid chromatography (HPLC)-NMR, HPLC-(mass spectrometry) MS and HPLC-MS-NMR methods. The principal metabolites were determined to be 2-hydroxyphenylacetic acid and 2-(2-hydroxyethyl)phenyl hydrogen sulfate, representing 24.3 ± 6.0% and 19.6 ± 6.4% of the dose, respectively. This indicates that metabolism of benzofuran to the polar species excreted in urine involves cleavage of the furan ring. The American Society for Pharmacology and Experimental Therapeutics ER -