PT - JOURNAL ARTICLE AU - Masaya Itoda AU - Yoshiro Saito AU - Akiko Soyama AU - Mayumi Saeki AU - Norie Murayama AU - Seiichi Ishida AU - Kimie Sai AU - Michiyo Nagano AU - Hiroshi Suzuki AU - Yuichi Sugiyama AU - Shogo Ozawa AU - Jun-ichi Sawada TI - Polymorphisms in the ABCC2 (cMOAT/MRP2) Gene Found in 72 Established Cell Lines Derived from Japanese Individuals: An Association between Single Nucleotide Polymorphisms in the 5′-Untranslated Region and Exon 28 AID - 10.1124/dmd.30.4.363 DP - 2002 Apr 01 TA - Drug Metabolism and Disposition PG - 363--364 VI - 30 IP - 4 4099 - http://dmd.aspetjournals.org/content/30/4/363.short 4100 - http://dmd.aspetjournals.org/content/30/4/363.full SO - Drug Metab Dispos2002 Apr 01; 30 AB - We found nucleotide variability in the 5′-upstream region and exonic sequences of a gene-encoding canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2 (cMOAT/MRP2) by polymerase chain reaction-based sequencing using genomic DNA from 72 established cell lines derived from 72 Japanese individuals. Four single nucleotide polymorphisms (SNPs) were found in the 5′-untranslational region and 21 in the exonic regions. Of them, 14 were nonsynonymous SNPs. One deletion of seven consecutive adenines resulting in a frameshift variant was also found. Four SNPs, c-24t, g1249a (V417I), c2366t (S789F), and c3972t (I1324I), were the same as those recently reported. A strong association was found between c-24t (5′-untranslated region) and c3972t (exon 28), with the promoter activity of the former worth being compared. The American Society for Pharmacology and Experimental Therapeutics